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The three modalities are complementary.

Immunofluorescence analysis of SLC25A39 in parental and SLC25A39 knockout HeLa cells treated with BSO (1 mM) and erastin (5 uM) for 24 h (bottom)

Right, volcano plot showing the relative fold change (log 2 ) in ER-metabolite abundance versus log 10 (P value) from Slc33a1 -knockout KP1 cells expressing a vector control or SLC33A1 cDNA

Caffeine and Behavior: Current Views and Research Trends